This band probably shows fecal contamination through microbial attacks or viral load

Kashif Ameer

this sample is preserved 99.5% Ethanol and stored -80°C in 4 months.

I can't understanding obtained band when sample were comtaminated microbial attacks. can you explain? and

1) because feces, microbial attacks are nature?

2) if this sample were comtaminated microbial attacks or something, could i diet analysis with this sample? (using universal primer)

thank you!

There are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can inhibit downstream PCR. The purpose of the study was to evaluate two different DNA extraction methods in order to choose the most efficient method for studying intestinal bacterial diversity using Denaturing Gradient Gel Electrophoresis (DGGE).

Prior to preservation of samples at 99.5% Ethanol and stored -80°C in 4 months, the microbial load might be present in the feces and this could be the reason of residual band.

Diet analysis with this sample using universal primer can be carried out for supplementary evidence.