Has anyone used Gibbs Sampling and Random Forest Statistical software? If yes please let me know the pros and cons?

Has anyone used Gibbs Sampling and Random Forest Statistical software? If yes please let me know the pros and cons?

07 August 2015 5,803 3 View

How can I overcome light bands in primer standardisation (gradient PCR)?

I am getting very light bands for Gradient PCR (Primer Annealing temperature for PCR).

11 December 2014 2,262 15 View

I want to get the positions of a gene or its regulatory elements in the given region. Can details regarding this be found anywhere in a websites/database?

I want take out all the genes falling under a given start and stop position of all the genes deletion/duplication. Is it possible to get that ? If its possible, then please let me know.

31 December 2012 8,243 1 View

Is there any site where if I submit the start and stop positions of a cytoband, I will get the entire genes falling in that region?

Extraction of genes present in a definite start and stop position.

31 December 2012 6,206 2 View

Thank you for answering the previous question. Can anybody can tell me what qualities are required for a good primer?

How can we check the specificity of a good primer?

04 May 2012 3,728 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View