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Questions related from George Perdrizet
Trying to get a steel plate to rust uniformly. Current strategy is as follows: abrade surface with steel wool, clean thoroughly with isopropanol, immerse in distilled water containing 1% H2O2 and...
07 July 2013 2,961 10 View
I am doing some in vitro RNA biochemistry experiments with a ~170 nt RNA. The RNA is a made up, model sequence. It has several 'weak spots' where, no matter how careful I am during...
04 April 2013 7,532 8 View
I am doing a RNA-RNA UV cross-linking experiment. The positive result I am hoping for is a cross-link between a 1.9 kb RNA and a 260-mer. This should produce a large gel shift on a denaturing gel....
01 January 2013 3,687 11 View
I would like a good estimate of protein content in ultracentrifuge fractions of crude cell extracts. I know nucleic acids are present in some of them (I can see it on a ethidium bromide stained...
10 October 2012 6,372 8 View
I am in the process of choosing several model test mRNAs for further study. This is being done based on simple analysis of some deep sequencing data and by bioinformatics using a few simple...
10 October 2012 7,017 2 View
Does anyone still do that? I do, and here is what happened. I am trying to enrich an 'activity of interest' from a human cell lysate 1960s style in hopes of identifying the responsible protein....
10 October 2012 4,610 3 View
