What are the ways to determine the IC50 in case of Antimicrobial and anticancer activities

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Al Munawir

Hi Kumar,

why do not you try GraphPad Prism software to calculate IC50 ?. www.graphpad.com/scientific-software/prism/

This is commercial software , but you can use the trial version .

good luck.

Prabhat Kumar Upadhyay

Thanks Munawir Sir, I am using Graph Pad but did not get that option, but i will try again.

Adam B Shapiro

Use SigmaPlot tofit the data to the Hill equation.

The XLfit add-in for Excel with equation 205 could also be used.

Antimicrobial MICs usually are step functions, however, so there is no point in trying to fit them. You just call the MIC as the lowest concentration tested that prevents growth in a set of 2-fold serial dilutions.