Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

How to isolate diazonium tetrafluoroborate ?

I'm trying to synthesis and isolate diazonium salt of tetrafluoroborate. this salt showing white color as per reported paper. while I'm doing after addition of NaNO2 colour showing faint yellow...

11 February 2020 4,590 2 View

How to calculate Eigen sensitivity matrix for structural dynamic problems?

I am solving finite element model updating problem. In order to achieve that i am studying inverse Eigen sensitivity method as a updating method. There, i am unable to understand how to calculate...

04 February 2020 4,513 4 View

Information extraction from unstructured documents?

I am looking for algorithm(s) to extract data and its value from unstructured documents. We were given list of tokens against which the information from the documents to be extracted. Going...

19 October 2017 9,065 3 View

How to calculate the amount of different phases in a composite?

Hi all, I have composites of magnesium silicate and magnesium titanate. I have confirmed from the XRD data that, they have different phases with the change in the composition. I want to calculate...

22 May 2016 1,566 2 View

How enhance transfection in CHO cells to express fusion proteins of approx. 90-100 KDa in size?

13 August 2015 5,668 4 View

Any recent or past work on anti tarnish coating on silver and copper?

I want to know conventional methods available to remove the tarnish as well as prevention methods of tarnish of metals.

22 December 2013 7,458 1 View

What is the role of the complexing agent in sol-gel process?

I have prepared two sols, one with complexing agent and other without complexing agent. Both the sols are stable and transperent, but when deposited films on glass substrate using dip coating, sol...

24 October 2013 7,753 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View