Howmuch time can we rely on the total igE test result for determining omalizumab dosing ?

there is a patient who has underwent a total igE test 1 month ago. can we rely on the results and determine omalizamab dosing? or the new test should be performed?

11 January 2021 253 2 View

Negative volume in LSDYNA?

I am modelling a hypervelocity impact in lsdyna software between a projectile and a wall. the projectile is made up of SPH particles and the wall is finite element. I faced an error related to the...

10 April 2020 9,351 3 View

Please introduce me a multi-class classification method?

28 May 2016 9,153 9 View

What DNA to use for making qPCR standard curve?

26 March 2016 8,209 8 View

How do I check the Host Cell Proteins of HighFive cells?

I am currently trying to purify proteins from different insect cell lines and baculoviruses. One of the criteria I want to check is the amount of host cell protein (HCP) in the purified sample....

28 August 2014 2,232 3 View

Does anyone have experience with a CsCl gradient not banding?

I have been trying to use a CsCl gradient to purify an intracellular virus. However after centrifuging at 100000xg for over 20 hours there are no bands and the cellular proteins are distributed...

05 July 2014 9,196 13 View

How can I determine overall volume of a protein from its crystal structure?

How can I determine overall volume of a protein from its 3D structure (x-ray crystal)? Could you help?

26 December 2013 9,899 9 View

Can someone advise on how to track metal release from metalloproteins?T

Looking for a method which is not so expensive

29 June 2013 1,686 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View