What is the assay buffer component in maleimide fluorimetric assay kit?

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What is the effective thickness of the Cellulose Acetate membrane?

I am currently engaged in modeling the desalination performance of Cellulose Acetate (CA)/Graphene Oxide (GO)/POSS Mixed Matrix Membranes (MMMs) for reverse osmosis (RO) applications. The primary...

18 August 2023 7,177 3 View

I have difficulties passaging human dermal fibroblast. How many passages can I continue routinely?

Human dermal fibroblast passage easily or human foreskin ones?

29 October 2022 1,378 4 View

What are the differences between human dermal fibroblasts and human foreskin fibroblasts in Research?

Which kinds of these cells (HFF or HDF) are better for wound healing research ?

29 October 2022 1,029 1 View

How can I write the research questions and hypothesizes in Structural Equation Modeling?

As there are 4 or 5 variables which I am trying to find the relations between.

13 January 2022 6,378 3 View

Looking for the appropriate constitutive models for fabrics and textiles cyclic loading?

The figure provided is the force/width vs strain measured for a piece of fabric in uniaxial stress configuration (the thickness is unimportant but you can assume what you like e.g. 1mm). The...

01 April 2021 8,054 1 View

Is it necessary to model material failure in quasi-static compression simulations?

Hi all, I am dealing with quasi-static compression simulations on topology-optimized lattice structures using the Abaqus Explicit solver. By the way, I have not modeled material failure model...

26 June 2020 3,707 6 View

Negative volume in LSDYNA?

I am modelling a hypervelocity impact in lsdyna software between a projectile and a wall. the projectile is made up of SPH particles and the wall is finite element. I faced an error related to the...

10 April 2020 9,649 3 View

Please introduce me a multi-class classification method?

Hi every one., I'm working on multi-class data, and I want to predict the test data. In this regard, I have problem with introduction the response matrix to the classifer. The most common methods...

28 May 2016 9,236 9 View

I am trying to isolate DNA from soil, however, I am not getting any bands from the fungal communities in my agarose gel, can anyone help?

hi I am working on soil microbial community structure, I isolated DNA from soil samples , when I do PCR with ITS-3-GC clamp and ITS-4 primers to study fungal community , there is no band in...

11 July 2015 10,008 5 View

How do I check the Host Cell Proteins of HighFive cells?

I am currently trying to purify proteins from different insect cell lines and baculoviruses. One of the criteria I want to check is the amount of host cell protein (HCP) in the purified sample....

28 August 2014 2,336 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View