We're looking to study the spheroid aggregation in a microfluidic device. The available literature either uses a lining of Matrigel (GFR type), or a cell lining of mesothelial peritoneal cells. We do not have access to the latter.
Are there any other considerations to keep in mind while designing this device, such that after the ovarian cancer cells are introduced via a continuous flow media, we should be getting spheroid aggregations ?
Also, we would be interested in being able to simulate to the maximum extent possible, the actual peritoneal cavity.
How would we get this done, without using the mesothelial peritoneal cell lining ?
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