Sreepadmanabh Mahadev

4 Questions 3 Answers 0 Followers

Questions related from Sreepadmanabh Mahadev

How do we increase the viscosity of a DMEM solution using Xanthan Gum ?

We have measured DMEM as having a viscosity of around 0.0035. For our experiments, we require to increase its viscosity to the range of 0.006 - 0.007. We were planning to use Xanthan gum in order...

05 May 2019 4,340 2 View

Why does a large peak corresponding to T appear at the very end of a Sanger sequencing chromatogram ?

While using Taq Polymerase for Sanger sequencing, does the addition of an extra A at the very end have a role to play in this observation ? And if so, why is the large peak observed only at the...

04 April 2019 340 3 View

What are the specific requirements of the channel lining in a microfluidic device to study ovarian cancer spheroid aggregation ?

We're looking to study the spheroid aggregation in a microfluidic device. The available literature either uses a lining of Matrigel (GFR type), or a cell lining of mesothelial peritoneal cells. We...

05 May 2018 2,647 0 View

Why is crude collagenase better at cell disaggregation than purified collagenase ?

Why is it not the other way round ?

11 November 2017 3,350 5 View