Recently, I tried to take some images of tube formation in angiogenesis using EA.HY 926 cells. I used Matrigel coated 96 well plate and D-glucose was used as the angiogenic factor. I noticed that focusing is not good in tube formed wells ( I used phase contrast microscope and the magnification was x10). Can anybody share your experience to overcome this problem.
Neelaka Molagoda
Dear Taleb,
Thank you very much for your response. 60 uL/96 well plate. I think, it is better to increase the Matrigel volume. I would pretty much appreciated if you can send the full text of the attached article. Good luck
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