Hi, you can use Giemsa stain then measure the differentiation using Image j. And for conformation differentiation, you can do immunocytochemistry using a differentiation marker.

Dear Sharif,

Thank you very much.

Dear Neelaka!

Please You also look at the protocol in article:

Article A quick, simple and unbiased method to quantify C2C12 myogen...

Dear Alexandr,

Thank you very much for sharing your knowledge. This article is really helpful.

Hi Neelaka, I hope you are very well.

Maybe this article is of interest to you, where FITC-Annexin V/propidium iodide (PI) staining assay was used to detect the number of primary apoptotic myoblasts

You can read it in: Article lnc9141-a and -b Play a Different Role in Bovine Myoblast Pr...

I hope it helps you.

Dear Maria,

Thank you very much for the information. I"ll go through the paper.