I prepare cell-derived matrices for my work. I have tried to dissolve it with few buffer options ( RIPA/ 30mM Acetic acid (ph 5)/ HBS EP+ (ph 6.2)/PBS (6.2)/ PBS(7.4)) but it didn't work well. I have followed a few papers where milling and pepsin digestion has been implemented, so I was planning to try once with that protocol but milling is not physible because of my sample size. So I am planning to sonicate the ECM as an alternate to milling the sample. I would like to request suggestions for the sonication parameters to try for dECM. My plan is to dissolve dECM and set it for regelation at 37 'C incubation. Help me and thank you.
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