Md Asrafuddoza Hazari

9 Questions 6 Answers 0 Followers

Questions related from Md Asrafuddoza Hazari

What are the sonication parameters to be tried to break the decellularized extra cellular matrix (dECM) network so it become soluble ?

I prepare cell-derived matrices for my work. I have tried to dissolve it with few buffer options ( RIPA/ 30mM Acetic acid (ph 5)/ HBS EP+ (ph 6.2)/PBS (6.2)/ PBS(7.4)) but it didn't work well. I...

24 April 2022 270 0 View

What should be the convenient approach for dissolving cell derived matrix ? (we will be using the same CDM solution for regelation)?

I would like to know the convenient ways by which we can dissolve the decellularised cell-derived matrix (CDM) and this same CDM solution we can use to form gel again after some period of...

06 April 2022 7,633 0 View

What could be an alternate of HFIP (Hexafluoro Isopropanol) solvent which is used for dissolving amyloid fibrils?

I use HFIP for dissolving hIAPP (Amylin) fibrils to make monomers. I would like to ask Which solvent could be a replacement for HFIP to dissolve amyloid fibrils?

11 May 2021 7,179 5 View

Servers for finding free energy and dissociation constant of protein-protein docked complex?

Hello everyone, I would like to get help about finding any server or application which can give me info about free energy and dissociation constant of protein-protein docking outputs. I have...

16 January 2021 867 3 View

I don't have 1kDa filter so am planning to pass a solution of 4kDa protein in 3kDa centricon. Please let me know about protein loss probability?

hIAPP monomer which is of 4 kDa. I want to do buffer exchange. is it fine to do with 3 kDa centricon filtration falcon?

15 January 2021 2,025 2 View

Is it necessary to minimize the pdb structure of proteins which are going to be blindly docked in servers like cluspro or interEVdock2(FRODOCK2)?

I am planning to do a docking of two proteins (one of which is 30KD and another one is 4KD). I would like to request the steps of protein protein blind docking. what are the online authentic...

16 December 2020 8,143 3 View

How to remove a unit from pdb file of a dimeric protein and saving it as monomer pdb file?

How to edit a pdb file of a dimeric protein so that dimer can be converted and saved as monomer pdb which can be used in docking experiment.

15 December 2020 1,772 4 View

How can I predict or measure the concentration of a protein in extra cellular matrix?

I want to measure the concentration or predict the range of concentration of insulin present in the ECM of pancreatic tissue. Or I want to measure the concentration of a particular protein at...

14 January 2020 5,571 2 View

How much rpm is suitable for 1.5/2 ml microcentrifuge tube?

I want to use it for ultracentrifugation at 55000 rpm. I have used the 1.5 ml microcentrifuge tubes at 19,000 rpm , it was fine then. I have a doubt about 55,000 rpm. Please suggest me with some info.

17 December 2019 1,376 3 View