I have roughly 200+ gene fragments that I need to get sequenced, but at 3.5$ a reaction (this is the price the core facility I use has set) the process will cost me upwards of 700$. Is there an alternative way to verify the quality of my gene fragments or perhaps do them for cheaper?
Chuck A Arize
Yes, there are cost-effective alternatives to Sanger sequencing for very large experiments. According to a source, Next-Generation Sequencing (NGS) is a popular alternative to Sanger sequencing. NGS is faster and more cost-effective than Sanger sequencing for large-scale experiments. It can also provide higher throughput and higher resolution data than Sanger sequencing.
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