Hi, I am sequencing a multicopy DNA region (rDNA-Sanger sequencing). I am interested in DNA polymorphisms (intra species - intra-individual). So I look for double signals (see in attached picture, green arrow). However, many times I notice here and there (not consistently) double signals appearing in the sequence generated by the forward primer but not in the sequence generated by the reverse primer or vice versa (see picture red arrows). Can somebody explain why these appear or what they are?

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