What are the secondary sequence signals generated by the forward primer but not the reverse, or vice versa, in rDNA-Sanger sequencing?

Hi, I am sequencing a multicopy DNA region (rDNA-Sanger sequencing). I am interested in DNA polymorphisms (intra species - intra-individual). So I look for double signals (see in attached picture,...

23 January 2015 5,827 5 View