Where can I get high resolution climatic data to study the suitability of habitat for a particular species?

What are the better remote sensors models to use to monitoring water quality?

28 December 2020 6,411 4 View

Who can share me otostegia integrifolia cultivation guideline or related material?

This is to know botany, ecology and cultivation practices.

09 October 2020 5,317 2 View

Does my title fit for a phd dissertation paper?

Project Partial replacement of waste glass and ceramic as coarse aggregate to produce c-25mpa concrete

22 December 2018 6,265 1 View

Which one is more critical: Food loss or Food waste?

This is for your valuable information for further planning our research work

28 October 2018 269 8 View

Which multiple mean comparison methods is the best way for 3 factor factorial design?

24 July 2017 3,550 3 View

Can you suggest resource on education for sustainable development for 6-8/9 years children?

I am particularly interested in exploring how to engage young children in sustainability issues? How can we maximize their learning of ESD issues? How can we create such a learning space?

20 August 2015 2,092 6 View

Role of essential oil in flower production?

Dear all, do you and any experience to share me with respect to essential oil utilization in cut flower handling?

01 January 1970 8,066 9 View

Authorship of the journal

I have a comfirmation letter of this Journal article from the joirnal editor! The autorship of the is mine! There was a mistake because of the same first and second name we had with Dr Kassahun...

01 January 1970 2,509 4 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View