What are the recommended primers for 16s sequencing analysis if I am interersted in species level in gut microbiota ?

06 November 2017 1 9K Report

I'm interested in analyzing gut microbiota compositions in a species level, so what is the recommended primers for that ?

Thanks !

Haitham Hajjo

Thank you !

Haitham

Badges
Science method

More Haitham Hajjo's questions See All

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

What is the significance of using Krasnoselskii's fixed point theorem in proving the existence of periodic solutions for impulsive neutral dynamic eq?

The significance of using Krasnoselskii's fixed point theorem in proving the existence of periodic solutions for impulsive neutral dynamic equations lies in its ability to provide a powerful...

18 April 2024 9,839 0 View

How can I preserve samples for DNA extraction and methylation analysis?

I want to preserve tissue samples to use them in DNA extraction for epigenetic and methylation analysis. How can I preserve samples for DNA extraction and methylation analysis?

21 November 2023 5,108 0 View

How calculate the thickness of ground patch antenna ?

how calculate the thickness of ground patch antenna ?

17 November 2023 4,017 3 View

How can i download CST Studio Suite with crack ?

I want a link to download the program

24 October 2023 9,495 3 View

How to correct green color of iron-ferrozine complex?

I am determining iron-chelating activity using Quercetin, but upon the addition of quercetin to the tube containing iron + Ferrozine, the color transforms into green which is far away from the...

02 October 2023 6,055 0 View

Iron-chelating activity determination?

Hi everybody I am working on iron-chelating activity using pure flavonoids (Quercetin and Rutin). I tried thiocyanate method for the determination of iron chelation but the complex formed with...

28 August 2023 6,337 0 View

How to import accident data in excel sheet to PTV Visum?

Dear Colleagues, I'm facing some issues trying to import accident data to PTV Visum. I would greatly appreciate your help.

13 August 2023 503 1 View

Is it possible to simulate Two-dimensional (2D) traffic flow using VISSIM?

Dear Friends and colleagues, Is it possible to simulate Two-dimensional (2D) traffic flow using VISSIM? If so, can you provide some methods and examples? Your help is highly appreciated. Kind...

17 July 2023 4,547 3 View

Dear Colleagues, does anyone have enough experience, or do you know someone who can share knowledge or give private classes related to Vissim?

Has knowledge of driving behaviour simulation, calibration, and model evaluation. Kind regards

15 April 2023 309 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View