Where can I find the scoring key for The Profile of Emotional Competence?

The author of this instrument has provided an email address in which to request a copy of the scoring key. However, I have attempted to contact him several times without success. I have been...

08 December 2019 5,289 1 View

Why am I unable to reproduce PCR genotyping results for a single colony that previously produced a band but now doesn't?

Previously I genotyped a 96-well plate containing genomic DNA from cell lysate. I was able to get a band at the desired location, however I am trying to reproduce the genotyping results for a...

28 August 2019 3,648 3 View

I am in need of a questionnaire on children's emotional development that is completed by their parent. Does anyone know of such an instrument?

I am looking for a survey or questionnaire on a child's emotional development that their parent completes. A survey/questionnaire on emotional growth, emotional competence, emotional well-being,...

03 May 2018 2,361 2 View

Current projects that involve collaboration between the arts and sciences?

I am looking for references to current projects that involve collaboration between the arts and sciences as part of my Future Fellowship research. If you are working in this area, or know of any...

02 February 2018 8,249 2 View

How trustworthy is evidence based research as applied in the natural sciences and the social sciences?

In medicine, I think evidence-based research and - crucial, this - evidence-based practice is quite robust. Insofar as social scientific veracity is concerned, the wide range of variables does...

06 September 2017 4,948 5 View

Does anyone have a preferred spectrophotometric detection method for arsenite vs. arsenate?

So far we have tried a variamine blue assay from Narayana et al. 2006 and a silver nitrate precipitation from Simeonova et al. 2004. In the variamine blue assay instead of intensity correlating...

29 June 2016 1,373 2 View

Order of minigene-GFP construct for IVT and electroporation for human dendritic cells?

We are developing a GFP construct to use as a marker for successful electroporation of a minigene into human dendritic cells. the purpose is to use the dendritic cells as potential targets for t...

28 January 2014 4,424 1 View

What is the best isotype for assessing background for cell surface activation/phenotyping markers by FACS?

I'm doing some extensive phenotyping by FACS of human lymphocyte populations. I'm looking at a number of markers- 41BB CD137 OX40 CD134 PD1 CTLA4 TIM3.... For many of these markers the isotype...

19 January 2014 8,114 3 View

What is the difference in effects of Bacteriophage and Antibiotics therapy?

Does anybody know of recent or current comparison studies between the effects of bacteriophage therapy and that with antibiotics?

03 October 2013 9,084 3 View

What is your favorite scientific paper of all time?

I am helping to teach a course on communicating science and through this course, I have come into contact with an absolutely beautiful paper. It is clear, concise, and elegant. I am not at all...

20 October 2012 558 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Multiple insertions with Q5 Site-directed mutagenesis?

03 February 2021 221 2 View