What are the reasons of jagged qRT-PCR fluorescence and melting curves ?

11 November 2015 0 10K Report

hi dear

i am working with human SNP resulting in two primer fragments please i need to know the reasons of jagged fluorescence and melting curves appeared occasionally during qPCR process , is that related to technical error, handling, software or instrumental problems, or something else ???

i am attaching some photos of my recent results

i will appreciate your help

thank you

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