How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why do results of NCBI Translated Blast (tblastn) and Standard Nucleotide Blast (blastn) differ if given the same sequence ?

I have an amino acid sequence If I run it on NCBI tblastn I get the expected results. But If I take the same amino acid sequence Obtain the nucleotide sequence of it using...

28 July 2020 7,676 4 View

All-against-all pairwise global alignments?

Hi all, I have a collection of nucleotide sequences corresponding to one functional domain of a hallmark gene of viruses. So in principle I am working with relatively-homogeneous functional...

28 March 2020 8,869 3 View

How do you interpret Blastn results with 0 % query coverage and 100 % identity?

I have a blasn result for a whole genome sequence of a species against a particular gene nucleotide sequence that encodes a protein. For these results the e-value is a bit high but I have 100 %...

05 November 2019 3,504 1 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

How to find out the non-aligned reads of a query fasta file consiting of sRNA sequences after running standalone blast against miRBase?

I am working with miRNAs in tea, and for finding out conserved and novel miRNAs, I have blasted the filtered data set of sRNA sequences against the miRBase. But the output only shows the aligned...

10 November 2018 7,704 5 View

Why no significant similarity found for a specific DNA sequence using Blastn?

Hi Everyone, I am working on finding the DNA sequence of the proteins with specific function. So far, I got around 5 proteins sequences which show a high similarity (> 96%). I use NCBI to...

21 October 2018 898 3 View

Why miRBase could predict the targeted miRNA?

In senior's previous paper,he use miRBase to predict the impact of SNP to miRNA binding.But miRBase main function is to use the name of the miRNA or use miRNA similar sequence to search the detail...

09 October 2018 315 2 View

What are acceptable thresholds when using BLASTn on fungal DNA sequences?

Hello, I'd like to know your opinions on identifying fungal DNA sequences using the BLASTn with regards to Max Score, Total Score, Query Cover, E-value, and Identity match %? What are common...

06 July 2018 9,896 1 View

Is there any tool to detect the presence of plasmids in fragmented genomes based on already assembled genomes (drafts included)?

Based on the lack of information from fragmented genomes, where there is not a correct description if the contig belongs to the bacterial chromosome or it comes from a plasmid, one option is to...

04 May 2018 1,672 1 View

Limited candidate miRNA, how do i increase my search for data mining ?

Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...

03 April 2018 1,826 2 View