Rolando Perdomo-Morales

3 Questions 4 Answers 0 Followers

Questions related from Rolando Perdomo-Morales

How can pronase-specific activity be determined using FITC-casein as a substrate?

Hi, I am determining pronase activity using a continuous fluorescence-based micro assay with FITC-casein as substrate (25 μg/ml final concentration in the assay). Excitation and emission...

16 May 2025 8,014 5 View

What are the proper query selection criteria for performing homology search of peptides (45 aa) using Blastn?

Hi, We are dealing with bioactive peptides of around 45 aa residues from crustaceans. I am planning to search for homolog peptides in other crustacean species using blastn. My doubt is whether...

03 March 2022 6,565 1 View

Does anyone could suggest a cosolvent or substance that increase solubility of poorly soluble drug while do not affect the intrinsic permeability?

The issue is we are trying to evaluate the intrinsic permeability of a molecule wich is poorly soluble (Class 2). However, many well-known solubilizers would also affect the permeability. I am...

07 September 2017 5,366 6 View