What are the principles behind viral plaque assays on mammalian cells?

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I wanted to shuttle a gene from a vector to another vector back bone. Question whether always whole ORF is necessary to clone?

The recepient vector has a already got two genes in it example say geneA and GeneB. Linked with P2A. i wanted to remove Gene B from the vector and replace it with another geneC. And removing geneB...

03 April 2016 490 2 View

What is the composition of cryopreservation medium for virus in -80?

Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...

07 May 2024 259 3 View

How freeze/thaw cycles affect virus culture fluid titers?

Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...

18 March 2024 5,174 4 View

What is the PCR technique used in Virology for the detection of viral nucleic acids for RNA and DNA viruses?

Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...

28 February 2024 1,869 10 View

May viral DNAs recombine with non-homologous recombination in eukaryotic cells (e.g., with non-homologous end joining)?

Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?

14 September 2023 7,245 3 View

RT step- Can I use 2 different target reverse primers instead of random hexamers? For 2 different PCR reactions on the same "first strand" RT product?

For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...

22 August 2023 1,600 1 View

Do you research project? Are you looking for a competitive candidate for your project?

I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.

26 July 2023 7,550 1 View

I am seeking of NZ2 strain ORFV virus. Is there the virus in your labs collection?

Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...

29 May 2023 7,105 6 View

Why HeraLabs supply piggybac transposase as a non autonomously replicating plasmid?

Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...

17 April 2023 5,873 6 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

What are the universal primer sequnces for H5N1 amplification by RT-PCR (for phylogenetic analysis purpose)?

Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.

27 November 2022 9,614 2 View