What are the possible sources of error if I run a gel and can only see the DNA ladder under the UV light?

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Comparing two chromatograms?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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Paul Rutland

Is this genomic dna or a pcr product being cut?

Is there a bright fluorescence caught in the wells where the samples were loaded?

Do you have a picture we can see?

How did you measure the amount of dna before cutting?

Adaobi Iwu

@Paul Rotland, It is plasmid DNA.

@Amy Johnson, thank you very much