Hello,

I need help in understanding the attached image. I did gene expression analysis to obtain DEGs in different groups of samples and extracted the upregulated genes in each. Next, I filtered out the genes upregulated in the liver stages ONLY i.e not shared with any other stages (liver-stage specific) and clustered the top 100 most variable genes from this list. Is it wrong for me to expect the highest peaks only in my LS samples?