I am trying to build a triculture model of the lung with type II alveolar epithelial cells (AEC's), lung fibroblasts, and conditioned media from lung macrophages. My goal is to keep the AEC component static, while changing fibroblast and macrophage treatments. The macrophages and fibroblasts would be primary cells, but I am hoping to use the cell line MLE15 in my triculture model because Type II AEC's are difficult to culture (in vitro they only keep their Type II phenotype for less than a week before turning into Type I) and again, I want the epithelial component controlled and unchanging from experiment to experiment.
Does anyone know of differences between the cell line and the primary cells that should prevent me from using the cell line in my model?
Type II alveolar epithelial cells are characterized by two functional and specific markers: LAMP3 (or DCLAMP) and surfactant.
In mice antibodies dclamp 1006F7, and SP1, bind very strongly to Type II AECs in IF, IHC and FACS.
It may be that the MLE15 cell line has maintained one of these particularities
Thanks! I know that MLE15 cells produce surfactant (at least SP-C), and a cursory glance at the literature seems to show that they produce LAMP3 too ... however they are a cancer cell line so I'm hoping I can find some justification to use them.
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