Agarose gel electrophoresis was run on samples of cDNA obtained from RT-PCR with specific primers, and scans of gel were analysed in Biorad.
I'm having trouble ascertaining whether the molecular weight estimated in the program is comparable to the desired molecular weight of the genes whose expression I'm analysing.
For eg. gene X is 511 bp, bands observed from two samples in gel is approx. 484 bp and 521 bp (when compared to MW ladder)
Can these values be comparable to the desired 511 bp and thus represent gene X or not?.
I understand they are observed as not the same molecular weight but to what degree can I chalk that up to how well the gel was loaded and run.