How we can grow the HT-29 cells on transwell for TEER? Is there any specific media we have to use??

Is there anything that we have to add in media for the differentiation of HT-29 cells or HT-29 MTX specific cell line or any differentiation inducer is used for TEER estimation in HT-29 cells??...

21 November 2020 7,243 1 View

Is this pandemic an outcome of globalization?

The societies around the world are fighting a battle to combat the further spread of Covid-19. This pandemic can be understood as a direct consequence of extensive globalization. Will this...

05 October 2020 258 3 View

Pls give suggestions about journal where article related to biomass( TGA ftir proximate analysis of seed) can get published soon?

Article includes characterization like TGA, FTIR and proximate analysis of 3 different types of seeds?

19 September 2020 3,455 3 View

Can anyone suggest which primer(s) (oligo dt/random hexamer or both) would be good to generate E. coli cDNA?

Knowing that very low percentage of E. coli mRNA is polyadenylated, perhaps random hexamer should also be used to generate even the 1st strand of cDNA, instead of oligo (dt) primer?

29 August 2020 6,515 3 View

Can i reuse Ni-NTA column for the same protein? Also, washing with 0.5 M NaOH didn't seem to work (as per the manual))?

Can i simply wash with MilliQ to wash off the imidazole from the column in order to run the second set of purification of the same protein??

14 December 2019 5,541 10 View

Have you used repertory grid interviews?

What have you used these for and where to learn about the various analysis that can be conducted using this method?

23 March 2019 8,369 2 View

Regarding NATIVE PAGE streaky pattern?

I purified my lectin from a seed by ion exchange chromatography followed by gel filtration chromatography.The eluted fractions after gel filtration chromatography were used to perform a...

23 August 2018 2,151 2 View

What is the best book to read hydrogen sorption mechanism in metals namely absorption,adsorption and diffusion?

Any Suggestions

08 July 2018 4,525 3 View

Is there any short term(0-3 months)course which can help with understanding Research Methodology & Data Analysis in depth?

This can be anywhere worldwide. It can be a certificate course or a summer school programme-but it must be on campus. If it concentrates especially on social sciences research or even management,...

20 March 2018 5,448 8 View

Hello everyone

19 May 2017 9,043 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View