Hello
Peroxidase activity 📷📷 Dormant seed Non dormant seed
17 April 2024 7,384 2 View
Our laboratory is currently investigating the impact of stress hormones on bacterial pathogens. Following the administration of stress hormone treatments, we conducted RNA sequencing analysis to...
14 December 2023 5,817 0 View
Hi, Could anyone please suggest appropriate bioinformatics tools to generate consensus sequence. I tried with EMBOSSCON but I am getting a number of 'n' in the consensus...
11 September 2023 7,619 1 View
I am working on skin coloration of koi carp.
05 July 2023 9,135 1 View
strigolactone for plants
29 June 2023 774 0 View
Information about 2D-DIGE
06 June 2023 6,748 2 View
In our latest study (Comparative assessment of sugarcane bagacillo and bagasse at... ) and even by the study conducted by Sluiter et al (https:// doi. org/ 10. 1021/ jf100 8023), it has been...
31 May 2023 2,510 0 View
I do LTP recording in rat hippocampus slices. I have been doing it for a while but off late I am facing a couple of strange issues. 1. The slice is thinning out with time while it is in the...
01 February 2023 2,491 3 View
Since lentiviruses are known to preferentially integrate into the transcriptionally active sites in the genome, I am wondering if there was a simple and effective strategy to identify clones that...
01 January 2023 1,418 2 View
I want to examine volatility spillover between Spot and future using EGARCH. The mean equation is as follows: retussi c retussi(-1) retusci(-1) Where retussi refers to spot and retusci refers to...
29 August 2022 4,325 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,405 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 949 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View
