Hello
Is there anything that we have to add in media for the differentiation of HT-29 cells or HT-29 MTX specific cell line or any differentiation inducer is used for TEER estimation in HT-29 cells??...
21 November 2020 7,243 1 View
The societies around the world are fighting a battle to combat the further spread of Covid-19. This pandemic can be understood as a direct consequence of extensive globalization. Will this...
05 October 2020 258 3 View
Article includes characterization like TGA, FTIR and proximate analysis of 3 different types of seeds?
19 September 2020 3,455 3 View
Knowing that very low percentage of E. coli mRNA is polyadenylated, perhaps random hexamer should also be used to generate even the 1st strand of cDNA, instead of oligo (dt) primer?
29 August 2020 6,515 3 View
Can i simply wash with MilliQ to wash off the imidazole from the column in order to run the second set of purification of the same protein??
14 December 2019 5,541 10 View
looking for a paid and fast (in terms of publishing) scopus indexed journal in physics or material science
17 June 2019 983 3 View
What have you used these for and where to learn about the various analysis that can be conducted using this method?
23 March 2019 8,369 2 View
I purified my lectin from a seed by ion exchange chromatography followed by gel filtration chromatography.The eluted fractions after gel filtration chromatography were used to perform a...
23 August 2018 2,151 2 View
Any Suggestions
08 July 2018 4,525 3 View
This can be anywhere worldwide. It can be a certificate course or a summer school programme-but it must be on campus. If it concentrates especially on social sciences research or even management,...
20 March 2018 5,448 8 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...
16 February 2021 1,808 1 View
