Hi fellow researchers, Does anyone have an idea as to where facilities are available for 2D-DIGE in Kerala or India?

More Deepti Madayi's questions See All

How we can grow the HT-29 cells on transwell for TEER? Is there any specific media we have to use??

Is there anything that we have to add in media for the differentiation of HT-29 cells or HT-29 MTX specific cell line or any differentiation inducer is used for TEER estimation in HT-29 cells??...

21 November 2020 7,243 1 View

Is this pandemic an outcome of globalization?

The societies around the world are fighting a battle to combat the further spread of Covid-19. This pandemic can be understood as a direct consequence of extensive globalization. Will this...

05 October 2020 258 3 View

Pls give suggestions about journal where article related to biomass( TGA ftir proximate analysis of seed) can get published soon?

Article includes characterization like TGA, FTIR and proximate analysis of 3 different types of seeds?

19 September 2020 3,455 3 View

Can anyone suggest which primer(s) (oligo dt/random hexamer or both) would be good to generate E. coli cDNA?

Knowing that very low percentage of E. coli mRNA is polyadenylated, perhaps random hexamer should also be used to generate even the 1st strand of cDNA, instead of oligo (dt) primer?

29 August 2020 6,515 3 View

Can i reuse Ni-NTA column for the same protein? Also, washing with 0.5 M NaOH didn't seem to work (as per the manual))?

Can i simply wash with MilliQ to wash off the imidazole from the column in order to run the second set of purification of the same protein??

14 December 2019 5,541 10 View

What are the paid scopus indexed journals in physics?

looking for a paid and fast (in terms of publishing) scopus indexed journal in physics or material science

17 June 2019 983 3 View

Have you used repertory grid interviews?

What have you used these for and where to learn about the various analysis that can be conducted using this method?

23 March 2019 8,369 2 View

Regarding NATIVE PAGE streaky pattern?

I purified my lectin from a seed by ion exchange chromatography followed by gel filtration chromatography.The eluted fractions after gel filtration chromatography were used to perform a...

23 August 2018 2,151 2 View

What is the best book to read hydrogen sorption mechanism in metals namely absorption,adsorption and diffusion?

Any Suggestions

08 July 2018 4,525 3 View

Is there any short term(0-3 months)course which can help with understanding Research Methodology & Data Analysis in depth?

This can be anywhere worldwide. It can be a certificate course or a summer school programme-but it must be on campus. If it concentrates especially on social sciences research or even management,...

20 March 2018 5,448 8 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

What is the best substrate for exfoliating few layers of hexagonal boron nitride (hBN) using traditional scotch tape method?

Unlike graphene, it is usually very difficult to exfoliate few layers (~10 nm thick) of hBN on SiO2 substrate due to lip-lip interaction at the edges. Au substrates have been proven very useful...

16 February 2021 2,635 2 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

How to prove the formation of a synthesis product using NMR ?

Hello, I am trying to identify the formation of a synthesis product. Knowing the precursors, I focused on the identification of the H-C long-range coupling between sites (a/b) and (a/c) using...

19 January 2021 8,438 5 View

What are the procedures for environment friendly and effective methods to prepare silicon QDs, Si nanotubes and silicene (2D materials)?

There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...

10 January 2021 3,418 1 View

How to conduct Methodological Research?

Hi everyone, I plan to create a design for a system using previously published articles, journals, etc as a basis. I found methodological research design but I don't know how to conduct it. Like...

07 January 2021 5,824 3 View

Qingzhong Wang

DIGE Analysis Software and Protein Identification Approaches - PubMed (nih.gov)

Steven Dodd

Hi Deepti Madayi

So from our records there are a few people/institutes in Kerala that have previously used our 2D analysis software so likely have (or did have) the facilities you require:

Mr. Vishal Kallappa Kavathekar at the T.D. Medical College Hospital, Vandanam, Alappuzha, Kerala

and

Dr. Pradeep Kumar G at the Rajiv Gandhi Centre for Biotechnology