Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Two different gaussian width in QM/MM Metadynamics?

Is it wise to use two different gaussians for two CVs during QM/MM metadynamics simulations?. Example for 0.5kcal/mol Gaussian height, For CV1 width is 0.3 and CV2 is 0.15. Where both CVs address...

10 March 2020 5,287 3 View

What is the impact of correlation test in Image encryption ?

Is correlation test sufficient for proving the efficiency of the encryption scheme?

23 February 2020 3,524 4 View

What is the significance of image entropy ( plain image and cipher image) in image processing?

Whether increase or decrease in entropy of cipher image is good for encryption? How?Why?

06 May 2019 9,299 12 View

Can PCR master mix solution be prepared in advance and stored at -20C?

For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....

27 January 2019 7,112 19 View

Can I stain cells with Annexin V after fixing with 70% ethanol?

I have treated cancer cells with inhibitors of cell cycle. In addition to looking at the stage of cell cycle they are in, I want to see if there is any apoptosis induced by the treatment. But I...

28 July 2018 1,242 3 View

How to measure the mesh size in COMSOL for PCF design for FEM analysis?

I want to know how to measure mesh size in COMSOL when are using initializing mesh than refined mode? Is that possible?

28 February 2018 4,418 3 View

Analytical formula of Natural Freqency of a composite with fixed-free (cantilever type) boundary condition?

I need to calculate the analytical relation for frequency of a cantilever composite beam, where one beam is prestressed on which an another non-prestressed beam is placed in a way to form the...

17 January 2018 2,773 9 View

What are the reasons for getting low value of R-square?

In my research I am getting a low value of R-square (0.253). What are the possible reasons for getting low value and How it can be interpreted?

16 September 2017 2,697 3 View

How can I give in ANSYS APDL, a load(in terms of displacement) such that an incremental strain rate of 0.001ps-1 can be applied?

I am trying to run a simulation in APDL, where I need to give incremental strain rate. I am giving a displacement of 5 units for a beam of length 10. How do we do it in incremental steps?

24 February 2017 2,189 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View