Especially to assay for inflammatory cytokines by ELISA in cell culture supernatant, what are the optimal conditions for storage?
How long can the media containing cytokines be stored in -80 deg C?
Jean-Jacques Pin
I think most of cytokines, chemokines and metalloproteases that contain the culture supernatant (lymphocytes for example) can be stored for decades at -80 ° C without altering their functionality.
I have a prélèvemment containing IL4, MIP3 (CCL20) and MMP12, which dates from 1994, which has not lost activity on the three molecules.
Obviously, this supernatant, which is the benchmark, were thawed once, because the loss is quickly dramatic every thawing / refreezing
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