They grow slowly and do not fill con-fluency!
This question has arisen in the section of the distillation experiment of soybean meal in animal feed.
27 August 2023 4,138 0 View
I have done the distillation of soybean meal and I want to calculate the percentage of soluble alcohol from the distillation.
26 August 2023 5,252 0 View
How can i calculate external radiation risk by DCAL software?
17 May 2023 2,677 3 View
I'm going to analyze wind flow in an urban area and the results of the velocity field are important. Envi-met looks good in thermal analysis and temperature measurement but minimizes the level of...
02 August 2021 7,311 5 View
I have seen in articles that only 4 elastic constants are provided for a material ( E1, E2,v12, and G12, besides the limit stresses of the material) like the image I put below. Assuming this is a...
07 March 2021 3,365 1 View
I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...
19 February 2021 1,199 6 View
I want a method to check if the surface I get is correct
24 December 2020 9,576 3 View
Hi I want to outline how to plot the phylogenetic tree for the housekeeping genes of Helicobacter pylori in the MLST method and to learn how to draw and interpret the treeThanks Jamal Falahi
12 January 2020 2,293 3 View
I am conducting a molecular dynamics simulation of a membrane protein channel, how can I calculate the diameter change of the pore which is critical for the transportation of ions during the...
02 December 2019 8,639 4 View
Computational costs are important in numerical methods, as if they were obtained by counting the number of operations(flops). The order to count this number has been asked by MATLAB that I have...
22 August 2019 3,255 8 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View