MLST interpretation and phyllogenetic tree ?

More Jamal Falahi's questions See All

Does anyone have a simulation code to calculate end of life for LFP batteries?

Hello, Everyone! I need a Python or Matlab code that calculates the cyclic and calendric aging of LFP batteries. There are numerous semi-empirical aging models for lithium-ion batteries, but I...

16 June 2024 1,861 2 View

How can I extract acetylcholine esterase enzyme from red blood cells through simplest way?

Dear Colleagues, I working on evaluation of choline esterase inhibitors activity,the problem that I don't have the enzyme. so i am trying to extract it from red blood cells of mice , rats ,rabbits...

30 April 2024 7,578 1 View

What is the effect of adding layers in a layered quantum well on the well depth ?

Hello everyone , for a layered quantum well, does the addition of layers in the quantum well increases its depth.

26 March 2024 3,757 1 View

Multiple Regression with low sample size (n<30 obs)?

Hello is there any advanced estimation technique to conduct multiple regression in the context of small sample size

09 March 2024 8,449 3 View

Hi, what is the endnote style for the journal "composite interfaces" published by Taylor and Francis?

Hi, what is the endnote style for the journal "composite interfaces" published by Taylor and Francis? Thanks

13 December 2023 6,184 2 View

Hi every one :can I evaluate the stability of polycaprolacton nano capsules via spectrometer . I dont have zeta apparatus or SEM ?

trying to prepare stable nano capsules by polycaprolactone so I need to observe the daily by simple way >

02 December 2023 9,238 3 View

Have you come across any accurate and reliable AI text detection software?

Dear Friends, I am searching for an accurate and reliable software tool that can detect whether a given text has been generated by an AI or written by a human. If anyone knows of such tools,...

30 November 2023 467 6 View

Hello everyone, Can somebody let me know how can i plot voltage gain in CST ?

I want to measure LC resonator's voltage Wirelessly

19 November 2023 4,077 3 View

Can XRD CS be bigger than TEM particle diameter?

Hi XRD and TEM experts It doesn't make sense to me but I still wanted to see if it's possible to have XRD crystallite size bigger than TEM particle diameter. I'm talking about high BET oxide...

31 October 2023 1,767 4 View

How to search in Google scholar with code?

How to search in Google scholar with code? I couldn’t figure out how to search in Google scholar with code. For example, I wanted an equivalent search for the following string: ( TITLE-ABS-KEY (...

20 October 2023 4,316 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Would you be willing to take a look at interview questions for a research study?

25 July 2024 6,938 3 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Brian Thomas Foley

The attached paper has a good description of both MLST and other genomics methods.

Harish Menghwar

Hi,

For reference MLST you can find MLST scheme on this link https://pubmlst.org/helicobacter/ You can also use the START. 2 software for phylogenetic tree or PHYLOVIz.

Best luck

Jamal Falahi

Tanks a lot dear coworker