I have been trying to improve CFU counts in some bench top fermentation experiments in the growth of Lactobacillus acidophilus.

I've tried a variety of media formulations starting with a basic MRS medium and exchanging various carbon sources like glucose and fructose and different nitrogen sources in ammonium citrate and glycine.

Has anyone tried adding things like glycerol or FOS or different amino acids?

Does anyone else have any experience in L. acidophilus media formulations or fermentation parameters?

Looking for any advice in increasing cell counts