29 April 2024 1 5K Report

How would you explain this in a 2500 word essay looking at critical theories to do with intersectionality and academic references

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How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

Do DH5a and MC4100 competent cells Fluoresce?

Hello, I have currently been tasked with performing a split GFP analysis. I have been using DH5a cells for plasmid transformation. The results were turning out to be what we expected. The...

29 May 2024 5,791 0 View

How do I prevent this deletion during ligation?

Hello, I am making some plasmid constructs via Inverse PCR and T4 enzyme ligation. I started with a plasmid that housed nucleotide sequences for two fused proteins. My goal was to cut out the...

14 May 2024 3,238 3 View

Krippendorff's alpha for two raters, many participants, with many many variables?

Hi all, We analyzed a qualitative dataset with short responses to 4 "fill in" items from over 250 people. We created 90 codes that can be applied across all the data (multiple can be applied to...

27 November 2023 2,154 0 View

Is there a macro for quantifying colocalization in a fluorescent microscopy pic in Image J?

I know there are a few different plugins you can use to quantify colocalization, but was wondering if anyone had helpful insight.

19 October 2023 1,147 1 View

What fixative is suitable for fixing microbial cells in suspension with minimal damage to DNA?

I am doing an experiment that requires me to fix some microbial cells, stain with antibodies, run through the flow cytometer and ultimately isolate DNA. The whole procedure is roughly 6 hours....

28 May 2023 8,512 1 View

IHC tissue sticking?

Never encountered this problem before (been a tech for 5 years), but here goes: Brain tissue derived from a perfused rat, stored in 4% PFA for roughly 4 days, then moved over to 30% sucrose in 1x...

19 April 2023 9,757 1 View

How to know if there is a significant different in likert responses?

I asked educators about their confidence levels (1=very unconfident to 5=very confident) in teaching 4 different topics: nutrition and health, social equity, environmental stewardship, and...

31 January 2023 2,655 3 View

How do you maintain primary murine enteroids through multiple passages?

Somehow this question ended up in the "Penicillin" category? So trying again. I am working with primary murine jejunal enteroids/spheroids, but I am having difficulty keeping them growing...

28 December 2022 6,807 0 View

How do you maintain primary murine enteroids through multiple passages?

This is going to be a long one. I am working with primary murine jejunal enteroids/spheroids, but I am having difficulty keeping them growing robustly past Passage #3. I would love some help!...

21 December 2022 3,018 0 View

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Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

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Are there any good simple systems or platforms to recommend?

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Why do exism movements become permanent dictatorship threats within liberal democracy thinking under majority rule-independent rule of law system?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

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