What are the optimal dilutions of nanoparticles for MTT assay on MCF-7 and MDA-MB-231 Cell lines?

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What are the recommended strategies for removing DNA contamination and preventing RNA degradation RNA extraction by TRIzol method?

I have performed RNA extraction of blood sample using TRIzol method. I ran RNA samples on non-denaturing gel (1.5%) that showed extreme DNA contamination and degraded RNA band. Please suggest me...

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After running RT-PCR for beta actin i got curves after 20 cycles. is this right? Is it mandatory that the curves should come within 20 cycles?

what is the reason behind this?is it due to the cDNA containation? usually how much cycles we are considering for doing PCR ?

06 December 2023 1,168 0 View

How to help to trouble shoot Agarose gel elctrophoresis?

I ve run AGE for cDNA but i got like this.what is the reason behind this?

13 November 2023 198 5 View

Why beta actin used as a house keeping gene for RT-PCR and western blot ?

what is the normal range in case of cell lines?

28 October 2023 3,309 3 View

During my cell culture after revival the cells were getting stressed .What might be the reason behind it ?

How long we have to trypsinize A549 Cell as well as Beas2b cell line?? is it due to to fault during cryopreservation ?

28 October 2023 6,894 1 View

For Beas 2b cell line can i use DMEM media for cell culture?

Is that ok to use DMEM media for maintaining beas2b cell line?

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Which is the best way for cell lysis during RNA isolation sonication or mechanical vortexing?

Does cell lysis affect RNA isolation? I ve done my cell lysis by vortexing after that i ve done RT but im not geting the results.

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Why we use normal lung epithelial cell line or MCF cell line as a control for the study of lung fibrosis ?

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24 September 2023 6,153 2 View

DLS of my xerogel samples show very high PDI value (>1)& very large standard deviation.Can I interpret any result out of this ? Or I need to repeat?

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How to isolate Neutrophils for the study of NETosis ?

Method to identify NETosis

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Adhesion strength of coating?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Hello researchers Is this a random laser or just fluorescence?

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Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

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What is the solvent for 8YSZ nano material?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Hugh J Byrne

It really depends on how toxic each one is, and for how long you wish to perform the assay.

For each, you should do a concentration range finding text (see 6.4. Concentration range of this guidance document)

https://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=ENV/JM/MONO(2018)19&doclanguage=en

It is worth thinking about what exposure time you want to study also:

Aleena Haqqi

Hugh J Byrne Thank you for responding