Dear,

After MTT, u need to do the following experiments:

1. Morphological study (Hoechst test, AO-PI, SEM, TEM)

2. Flow cytometry (Annexin V, Cell cycle, TUNEL)

3. Caspase activity

4. Western blot

5. PCR

this paper will help u too much, TQ

Article Zerumbone-loaded nanostructured lipid carrier induces G2/M c...

Dear,

After MTT, u need to do the following experiments:

1. Morphological study (Hoechst test, AO-PI, SEM, TEM)

2. Flow cytometry (Annexin V, Cell cycle, TUNEL)

3. Caspase activity

4. Western blot

5. PCR

this paper will help u too much, TQ

Article Zerumbone-loaded nanostructured lipid carrier induces G2/M c...

Pcr

Why pcr?

Yes, i had already done MTT Assay for my traditionally prepared drug. In MTT it shows 85% Inhibition. What you think about the gene expression study?

Hi,

 You can go for

1. Trypan blue exclusion assay

2. LDH assay

3. Staining live and dead cells (Hoechst, PI) using confocal / Flurorescence microscopy

4. Cell cycle analysis using flow cytometry

5. ROS detection by DCF-DA and NOS measurement

6. Expression of caspases by western blots and RT-PCR

Hope this helps!

Thank you