I need the brief details regarding the estimation of EGCG. Which type of analysis should I follow?
Yes, there are so many methods for total phenolics. For specific method EGCG, you can follow HPLC method.
You can follow our paper
Biochemical attributes of tea flowers (Camellia sinensis) at different developmental stages in the Kangra region of India
Cayman has a very good commercial kit ( Item # 10011096) for cell based EGCG assay. For plasma EGCG yo can use LC-MS with negative-ion electrospray ionization as described in the Clin Ther. 2012;34:2279–2285 article.
I am sharing with you the Cayman product specification sheet and the Clin Ther article as a single combined pdf.
Hope that helps.
There are a number of methods by which EGCG can be identified and quantified specifically. It really depends on how much amount of EGCG is present in your samples. For example, if you want to quantify the content of EGCG from some vegetable and/or fruit based samples, what you need to do first is an effective extraction process to concentrate EGCG amount in your sample. For the identification and quantification, a HPLC method would be the best for you to choose. However, if the sample you measure is not a pure solution, you may need EGCG standard for tentative identification by comparison with retention time and max absorption wavelength.
In another hand, if the trace amount of EGCG is present in your sample. a HPLC-MS technology will help you out effectively. Due to the detection limit of DAD detector on HPLC, you have to quantify EGCG using MS chromatogram via MRM mode.
Hope this would help,
(Zheng) James
If you want to quantify EGCG alone, you need to isolate and purify the sample using chromatographic technique, then you can use direct spectroscopic method to quantify EGCG. If your sample contains other compounds then better use HPLC method for quantification of EGCG (ISO14502-2).
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology