What are the most reliable tools or softwares for designing of the CRISPR gRNAs?

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Kasidet Hiranniramol

Some additional tools I can recommend:

WU-CRISPR - http://crispr.wustl.edu/

CRISPR ML - https://crispr.ml/

CRISPRScan - http://www.crisprscan.org/

Notably, CRISPRScan was trained using sgRNAs expressed from a T7 promotor while the other 2 tools were trained using sgRNAs expressed from a U6 promotor.

Samira Rahimirad

Thanks for all precious answers