What is the best way for mobility estimation in GROMACS?

I need to estimate the mobility of some atoms in the molecule within a molecular dynamics simulation and compare the values for different simulations. I use trjconv -fit rot+trans to fit C-alpha...

19 January 2021 531 3 View

How can one calculate difference between normal pseudo-solution and exact one?

Dear collegs, Let us have a linear system: Ax=b, (1) A is a matrix of (N,N) shape, b, x are vectors of N shape. We decrease number of equations to form a underdetermined system: A'x=b', (2) A' is...

11 November 2020 8,549 2 View

How is theoretical proportion of the total organic carbon of PLA (polylacitc acid) ?

Dear Sirs, I have measured the proportion of the total organic carbon (TOC) of polylactic acid (PLA) method DIN 19539. The results are 35,1%. Is it correct? How is the theoretical proportion of...

04 November 2020 6,598 1 View

Is there any monthly or bi-monthly 'free' Scopus or SCI indexed journal?

Looking for a fast-track journal to publish one of my papers in the field of image processing for non-hodgkin leukemia cancer cells detection method.

02 November 2020 5,289 3 View

How long do the antibacterial coatings work?

Dear all, I am looking for publications dealing with the long-term stability of antibacterial coatings. I need to know if the coating still works even after months or years. The latest term I...

06 October 2020 1,726 5 View

Someone has replaced the abstracts of my papers by entirely wrong texts. How can I correct this?

This appears to be a pure sabotage. Someone has thrown out my abstract - so far I discovered two - about scarce rationality, and about institutions in evolutionary economics - and replaced...

17 September 2020 1,994 3 View

How to statisticaly analyze distribution which is very skewed to low values (like Pareto distribution or similar)?

I have results of porosity analysis from CT data which is a very skewed distribution (looks like Pareto distribution or similar). I have data from a few samples which I want to compare about the...

14 May 2020 9,595 9 View

Which social media monitoring system do you prefer in your country?

Hello, colleagues! Which social media monitoring / listening system do you prefer in your countries? In Russia we mostly use Brand Analytics and Youscan.

23 January 2020 4,619 7 View

Why Antennapedia Control peptide inhibits the reactive oxygen species production in human blood?

Hi all, I use MyD88 Inhibitory Peptide (more details here: https://www.novusbio.com/products/myd88-inhibitor_nbp2-29328#datasheet or https://www.invivogen.com/pepinh-myd#about) to inhibit the...

04 January 2020 2,207 6 View

How to estimate a quality of single tracks fabricated by selective laser melting of a new Al-Zn-Mg alloy?

Dear friends, We approached toward SLM of a new Al-Zn-Mg alloy using single track experiment. The initial feedstock powder was ball-milled (not atomized). More than 10 tracks fabricated at...

05 November 2019 7,073 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View