I would like to prepare filamentous fungal biomass (Fusarium solani) to get a mycelium for biodegradation experiment. If anybody suggest me, would be useful.
Thanks in advance
Hi! You can inoculate the spores in sabouraud dextrose agar and then incubate at 30ºC. I think the mycelium growth should occur in a week.
If you have a cork borer you can use an agar plug from the plate you are growing them. It's a semiquantitative method but it's usually reproducible if you always use the same solid media, culturing conditions and age of the source solid culture. By the way, always take the plug from the border of the colony.
Otherwise you can try counting spores.
Thank you very much Xabier Vázquez Campos for your suggestion. If i use inoculum from solid medium, it would be a possibility to get agar when i check the dry weight after incubation.
I have worked with Fusarium flocciferum mycelium for biodegradation studies for many years; for biomass preparation, as mycelium or spores you can read my papers. If you have any doubt please feel free to ask.
Thank you very much Prof.Ana Maria Anselmo for your kind help. Would you mind, May i get your E.mail id.
hi, check it
http://link.springer.com/article/10.1007/s10924-011-0367-4
thank you very much Dr.George but I could not find the procedure about Fusarium sp.
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