I want to understand the specific elements influencing the distribution of tularemia in the region, helping researchers and public health officials devise targeted control and prevention strategies for tularemia( Francisella tularensis).
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...
28 July 2024 5,410 1 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field
02 July 2024 4,031 3 View
i have attached my model and i have used point based mesh independent fastener using attachment points and type of connector is basic cartesian but this is not working as i apply displacement on...
02 July 2024 6,293 0 View
Also, explain its pros and cons concerning other organic sources of nutrients.
01 July 2024 612 0 View
I have stocked my laboratory strain of Francisella (F. tularensis sub sp. holarctica LVS) in 100 % glycerol (800 microliter bacterial culture + 200 microliter 100% glycerol ) at -80C refrigerator....
05 July 2018 4,996 2 View
That is a frequent question concerning Ixodes ricinus asked by public. And there are two groups of parasitologists answering - the ones who say "maybe" and the others who say "have some doubts"....
04 June 2018 2,611 2 View
I am planning to make mutant in Francisella novicida using suicidal vector. For designing primer set i need clear conception about principle of action of suicidal vectors. Can anybody send me the...
26 July 2016 1,015 2 View
I'm trying to get a good protocol for PCR on tick DNA extractions for Francisella tularensis. Does anybody have any good suggestions? I've seen a couple but mostly multiplex reactions, and nothing...
21 January 2016 3,792 3 View
31 December 2015 5,469 4 View
Hi all , It may be basic question but Iam in urgent need to answer , for people who work too much with Tilapia and specially regarding to immunity, which age and size Tilapia can start develop...
12 November 2015 3,104 2 View
Dear Colleague, The Research Centre for Emerging and Reemerging infectious diseases of Pasteur Institute of Iran and Centre for Communicable Diseases Control and Prevention in Iran Ministry of...
09 October 2015 4,314 0 View
Hi all , I want to grew francisella in any media that doesnot contain animal bi-products , I usualy use cystein heart agar with adding 2% of bovine haemoglobin , but uregently I want to grow with...
23 August 2015 4,068 3 View
07 August 2015 5,632 3 View
I've been infected bone marrow derived macrophages for years now with various strains of Francisella tularensis but the cells will not become infected anymore. I use bone marrow derived...
05 June 2015 6,796 8 View