Which primer pair is better for amplifying endophytic fungal communities in roots of trees while avoiding host amplification?

Which primer pair is better for amplifying endophytic fungal communities (metabarcoding with Illumina sequencing) in roots of trees while avoiding host amplification? I would greatly appreciate a...

03 September 2020 728 3 View

No title

Hello, I am looking for the original version of COHIP. To measure quality of life related to oral health, in children.

15 June 2020 5,166 1 View

What is the correct interpretation for this association between changes in two variables?

Hello everybody! We are carrying out a research where we associate baseline to post-intervention changes between motivation and physical activity variables. My question is the following: In an...

24 March 2020 910 3 View

UMBRELLA SAMPLING: How can I calculate dG from a PMF curve in automated fashion?

1. I am processing my umbrella sampling simulations using the Weighted Histogram Analysis Method in Gromacs (gmx wham). However, I would like to report the mean dG and a standard deviation. To do...

15 March 2020 1,455 3 View

How do I calculate free energy of binding for ligand-protein complex using Bennett Acceptance Ratio (BAR)?

Hi, I am familiar with performing MD simulations using Gromacs on protein-ligand complexes. However, BAR is a new technique to me and I need specific clarification of the decoupling sequence...

12 March 2020 3,619 1 View

What are the most obvious difficulties when we expose the scientific findings to the general population?

When we have the opportunity to express our scientific findings in front of our colleagues we have the opportunity to talk with similar people, but; What happens when we have to speak in front of...

13 January 2020 501 4 View

Overlap PCR resulting into smear. Any idea of why?

I want to fuse 2 genes (4kbp each gene). I amplified both of them by PCR adding an overlapping region of 27 bp and gel extracted them. First thing that I don't like is that I can't quantify the...

17 December 2019 6,622 9 View

Where can I get a key to Saturniidae (silkmoths) genera?

I would like to know if there are taxonomic keys, besides the ones in Lemaire's books, for any group within Saturniidae family. UPDATE: Thank you all for your answers, Lemaire's books are indeed...

22 May 2019 2,124 15 View

What are the most important functions to know in SPSS for Psychology?

I am on a short deadline and need to learn how to use spss. The program is very large and I am unsure where I should spend my time the most. Chi-square & T-tests Correlations &...

21 May 2019 9,279 2 View

What statistical software are being recommended lately to carry out the confirmatory analysis of a questionnaire?

There are different software to carry out confirmatory analyzes, such as SPSS AMOS, which sometimes are insufficient for the publication requirements of some journals. What would be the most...

11 March 2019 5,216 13 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View