How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View

Why am I getting inconsistent results from my ChIP PCR?

One of the times I performed this ChIP I got strong binding at the locus I amplified, but I have not been able to reproduce this result after many many tries. However, I did get the protein I am...

10 December 2020 7,279 1 View

Do you know of articles that compare repatriates who return home after time abroad (experimental grp) with a stay-at-home group (control grp)?

It can be on any dependent variable and the sample can be of any age group.

03 October 2015 6,686 4 View

Are you aware of any studies that address validity of self-report attachment instruments?

I am a fan of the AAI and the developers on the CAI, so I need to counterbalance my positive bias with some critical questions about validity.

16 November 2013 5,733 3 View

Can the coefficient of variation (CV) of ANPP exceed 1 (i.e. 100%)?

I'm running a simulation on effects of changing mean and SD of rainfall on mean and CV of ANPP. At the most arid site I'm getting values of >100% CV for ANPP, when simulated mean rainfall is...

28 July 2013 814 8 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

A three electrode sensor inkjet printed gets damaged in few hours of usage in a dynamic media flow chip, any solution for that?

We are using some biosensors which are inkjet printed and are used in the microfluidic chip where media is flowing at the flow rate of around 60 uL/min. However, after the continuous use of few...

05 January 2021 610 2 View

Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

How to immobolise streptavidin to polyacrylamide using EDC/NHS?

Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...

17 December 2020 7,944 2 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View