Pertaining to the distribution environment.
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
Hi all, We have been trying to figure out why we see such differences in our base pair (bp) fragment size between doing a 1-step PCR reaction vs a 2-step PCR reaction to prepare metabarcoding...
20 December 2023 7,616 0 View
I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...
14 February 2021 5,960 1 View
One of the times I performed this ChIP I got strong binding at the locus I amplified, but I have not been able to reproduce this result after many many tries. However, I did get the protein I am...
10 December 2020 7,510 1 View
I have performed a ChIP (on 15cm plates) by making a fresh PFA solution, adding it to a 1% final concentration to my cells for 10 minutes and neutralizing the PFA with 2.0M glycine added to a...
21 November 2020 708 2 View
I am hoping to crosslink the protein-protein interactions in cells as well as the protein-DNA interactions (for which I use formaldehyde). Has anyone tried using the crosslinker DSS for this purpose?
03 August 2020 2,030 2 View
I am 3d printing gelatin hydrogel with fibroblasts embedded and I would like to access how long the fibroblasts remain alive in culture/ how long they retain their functionality. I plan to perform...
17 June 2020 5,022 1 View
I ran a 5-15% gradient gel that I handmade and ran it overnight. I also left an empty lane between each sample and they still managed to spread into one another. I loaded 170 ug of sample (I know...
14 May 2019 8,915 5 View
I am immunoprecipitating a protein for analysis by mass spect and I was told to minimize tris and also PEG for a clear analysis by mass spectrometry. I lysed in RIPA buffer with 50 mM tris and 1%...
15 April 2019 8,139 0 View
Recently our lab has started a project where we are trying to capture non-coding RNAs (
25 January 2017 5,258 13 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...
28 July 2024 5,771 2 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I am looking to purchase a photography kit for onsite metallurgical investigations, such as capturing photos of worn areas, hot tears, cold tears etc. Could anyone suggest me a good...
22 July 2024 3,490 2 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
I want to reduce simulation time in CST for frequency domain solver by upgrading my computer. I already have discrete GPU but it doesn't seem to make any difference at all compared to integreted...
10 July 2024 2,789 4 View
