What are the limitations when you use CRIPSR/Cas or dCas target a specific sites studying gene function and doing epigenetic modification?

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Request Python code?

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Ayoob Obaid Alfalahi

Dear Wei...

Will you check this link:

Chapter CRISPR/Cas9: a versatile genome-editing tool for crop improvement

Also, Try to reach Dr.Yuan the author.

Best Luck

Wei Li

I wanna find limitations of mammal cell targeting in vivo and vitro.

Unless you don’t stably transfect your cells, those Epigenetics changes are erased after a while

So how to stably transfect your cells？（improve the stability of plasmid or insert sgRNAs, dCas9-Suntag in Rosa26 loci).

By the way, do any people investigate what effectors cause epigenetic modification? I assume that epigenetic modification could result from inactivation of epigenetics-effector/protein or gene mutation in some loci unless why epigenetic modification is dynamic and the loci that have been changed revert into previous situation again.