17 Questions 9 Answers 0 Followers
Questions related from Wei Li
e.g. lentivirus can be used in delivering of gene of interest into both dividing or non-dividing cells, while gamma-retrovirus is limited to dividing cell. In mixture population of non-dividing...
23 August 2018 4,786 2 View
Except Addgene plasmid 101
12 May 2018 8,046 3 View
I think it's because of internal reasons (e.g. temperature-sensitive ori) in plasmid itself and natural reasons (consume lots of resources in cells and probably digested by protease). In terms of...
12 May 2018 3,241 1 View
Limitation: 1) target specific gene: 2) do epigenetic modification:
18 March 2018 3,336 3 View
Could you categorize the types of diseases according to changes of gene sequence, gene modification, others.
10 February 2018 7,433 1 View
the spot in microchip is quite small, so I guess fluorescence data in 96-well plates after being detected by Fluorescence Detector also need to be corrected using some ways of data handling
04 February 2018 659 2 View
add all numbers outside of the bracket in "matches"? like 1+1+1+1+1+.....
30 January 2018 2,340 4 View
could take an example in the below picture.
30 January 2018 7,718 3 View
How to relatively accurately quantify the SDS-PAGE gel and DNA electrophoresis gel? Any methods?
27 January 2018 7,234 3 View
e.g. I have a set of GFP-based membrane proteins, I detect the fluorescence intensity. I could say the more intense detected fluorescence, the more the amount of fusion protein. But what is the...
26 January 2018 539 4 View
Experiment: express a simple protein (e.g. membrane protein of E. coli) in E. coli in a way of fusing GFP and membrane protein. Linear? or exponential or else ??
22 January 2018 2,232 3 View
As shown in this picture, if the rate of protein sequence coverage is a result that the number of matched amino acids among MS/MS peptides is divided by the total number of amino acids in searched...
19 January 2018 3,843 1 View
it seems many senario that we have to/could do log transformation) e.g. make growth curve of bacteria, OD650 values could be transformed into corresponding log value, then plot growth curve. When...
02 January 2018 6,897 4 View
include ethic part, but not limited to this. mainly in operation and technology Is there any hindrance to this
23 October 2017 3,143 3 View
Can I calculate it? if I can, how to calculate? if I lack of conditions? Or Why I cannot?
10 October 2017 3,410 2 View
A chemical question in fact.
10 October 2017 5,347 1 View
Can you list any differences of protein production by animal cell culture and microbial culture?
08 October 2017 7,700 4 View
