I want to isolate metagenomic DNA from different plant tissues of cucumber internally, so for that which Kit I can use and if I follow any manual protocol which is the best one?
Dear Navya,
My lab routinely uses bead beating coupled with flocculation (if you want commercial kit: MoBio/Qiagen Powersoil DNeasy if I remember well, there are also many possible replacements). It works well on basically everything (soil, feces, roots, leaves, seeds, etc.) and if you assemble your kit yourself, it's much cheaper (~25% of the kit cost). The only drawback is the need for a decent bead beater (PowerLyzer, FastPrep etc., PL is the best I've worked with). If you are interested, I can share our protocols with you.
Best regards,
Marcin Golebiewski
Dear Marcin Golebiewski,
I am very interested to study your protocols, please kindly share with me. Thank you very much Marcin Golebiewski .
Dear Navya,
Please find the protocol in the attachment.
Best regards,
Marcin Golebiewski
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