I want to isolate the following genus prevotella, bacillus, and streptomyces from the seed and leaf samples. Is there any selective media to isolate only those and avoid others?
Mannitol yolk polymyxin B agar (MYPA) and polymyxin pyruvate egg yolk mannitol bromothymol blue agar (PEMBA) are selective media for Bacillus genus
Prevotella genus (Cultivation of the gut bacterium Prevotella copri DSM 18205T using glucose and xylose as carbon sources. 2021. Fang Huang, Roya R. R. Sardari, Andrius Jasilionis, Olof Böök, Rickard Öste, Ana Rascón, Lovisa Heyman-Lindén, Olle Holst, Eva Nordberg Karlsson)Article Cultivation of the gut bacterium Prevotella copri DSM 18205T...
Streptomyces genus (Oatmeal-agar, MYM-agar and ISP4-agar media).
Dear, Navya,
The medium PEMBA which consists of polymyxin pyruvate, egg yolk, mannitol bromothymol blue and agar, is a suitable medium for isolation Bacillus spp.
The bacterium Prevotella copri has formerly been recorded to be highly sensitive to oxygen, therefore it's difficult to isolate easily. But the medium PYX (Peptone Yeast Xylose medium) can be used successfully to isolate it. it can give a good growth on it.
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